Experimental human metapneumovirus infection of cynomolgus macaques (Macaca fascicularis) results in virus replication in ciliated epithelial cells and pneumocytes with associated lesions throughout the respiratory tract
Identifieur interne : 005B38 ( Main/Exploration ); précédent : 005B37; suivant : 005B39Experimental human metapneumovirus infection of cynomolgus macaques (Macaca fascicularis) results in virus replication in ciliated epithelial cells and pneumocytes with associated lesions throughout the respiratory tract
Auteurs : Thijs Kuiken [Pays-Bas] ; Bernadette G. Van Den Hoogen [Pays-Bas] ; Debby A. J. Van Riel [Pays-Bas] ; Jon D. Laman [Pays-Bas] ; Geert Van Amerongen [Pays-Bas] ; Leo Sprong [Pays-Bas] ; Ron A. M. Fouchier [Pays-Bas] ; Albert D. M. E. Osterhaus [Pays-Bas]Source :
- The American journal of pathology [ 0002-9440 ] ; 2004.
Descripteurs français
- KwdFr :
- Animaux, Bronches (anatomopathologie), Bronches (virologie), Cellules épithéliales (ultrastructure), Cellules épithéliales (virologie), Cils vibratiles (virologie), Humains, Infections de l'appareil respiratoire (anatomopathologie), Infections de l'appareil respiratoire (physiopathologie), Infections de l'appareil respiratoire (virologie), Infections à Paramyxoviridae (anatomopathologie), Infections à Paramyxoviridae (physiopathologie), Infections à Paramyxoviridae (virologie), Macaca fascicularis, Metapneumovirus (isolement et purification), Metapneumovirus (physiologie), Modèles animaux de maladie humaine, Poumon (anatomopathologie), Poumon (virologie), Réplication virale (physiologie), Trachée (anatomopathologie), Trachée (virologie).
- MESH :
- anatomopathologie : Bronches, Infections de l'appareil respiratoire, Infections à Paramyxoviridae, Poumon, Trachée.
- isolement et purification : Metapneumovirus.
- physiologie : Metapneumovirus, Réplication virale.
- physiopathologie : Infections de l'appareil respiratoire, Infections à Paramyxoviridae.
- virologie : Animaux, Bronches, Cellules épithéliales, Cils vibratiles, Humains, Infections de l'appareil respiratoire, Infections à Paramyxoviridae, Macaca fascicularis, Modèles animaux de maladie humaine, Poumon, Trachée.
- Pascal (Inist)
- Wicri :
- topic : Homme, Singe, Association, Médecine.
English descriptors
- KwdEn :
- Animals, Association, Bronchi (pathology), Bronchi (virology), Cilia (virology), Ciliated cell, Disease Models, Animal, Epithelial Cells (ultrastructure), Epithelial Cells (virology), Epithelial cell, Experimental study, Human, Humans, Infection, Lesion, Lung (pathology), Lung (virology), Macaca fascicularis, Medicine, Metapneumovirus (isolation & purification), Metapneumovirus (physiology), Monkey, Paramyxoviridae Infections (pathology), Paramyxoviridae Infections (physiopathology), Paramyxoviridae Infections (virology), Pneumocyte, Replication, Respiratory Tract Infections (pathology), Respiratory Tract Infections (physiopathology), Respiratory Tract Infections (virology), Respiratory tract, Trachea (pathology), Trachea (virology), Virus, Virus Replication (physiology).
- MESH :
- isolation & purification : Metapneumovirus.
- pathology : Bronchi, Lung, Paramyxoviridae Infections, Respiratory Tract Infections, Trachea.
- physiology : Metapneumovirus, Virus Replication.
- physiopathology : Paramyxoviridae Infections, Respiratory Tract Infections.
- ultrastructure : Epithelial Cells.
- virology : Bronchi, Cilia, Epithelial Cells, Lung, Paramyxoviridae Infections, Respiratory Tract Infections, Trachea.
- Animals, Disease Models, Animal, Humans, Macaca fascicularis.
Abstract
A substantial proportion of hitherto unexplained respiratory tract illnesses is associated with human metapneumovirus (hMPV) infection. This virus also was found in patients with severe acute respiratory syndrome (SARS). To determine the dynamics and associated lesions of hMPV infection, six cynomolgus macaques (Macaca fascicularis) were inoculated with hMPV and examined by pathological and virological assays. They were euthanized at 5 (n = 2) or 9 (n = 2) days post-infection (dpi), or monitored until 14 dpi (n = 2). Viral excretion peaked at 4 dpi and decreased to zero by 10 dpi. Viral replication was restricted to the respiratory tract and associated with minimal to mild, multi-focal erosive and inflammatory changes in conducting airways, and increased numbers of macrophages in alveoli. Viral expression was seen mainly at the apical surface of ciliated epithelial cells throughout the respiratory tract, and less frequently in type 1 pneumocytes and alveolar macrophages. Both cell tropism and respiratory lesions were distinct from those of SARS-associated coronavirus infection, excluding hMPV as the primary cause of SARS. This study demonstrates that hMPV is a respiratory pathogen and indicates that viral replication is short-lived, polarized to the apical surface, and occurs primarily in ciliated respiratory epithelial cells.
Url:
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PascalFrancis, to step Corpus: 000875
- to stream PascalFrancis, to step Curation: 000115
- to stream PascalFrancis, to step Checkpoint: 000849
- to stream Main, to step Merge: 006000
- to stream Pmc, to step Corpus: 000822
- to stream Pmc, to step Curation: 000822
- to stream Pmc, to step Checkpoint: 001666
- to stream PubMed, to step Corpus: 002D81
- to stream PubMed, to step Curation: 002D81
- to stream PubMed, to step Checkpoint: 002D09
- to stream Ncbi, to step Merge: 000829
- to stream Ncbi, to step Curation: 000829
- to stream Ncbi, to step Checkpoint: 000829
- to stream Main, to step Merge: 005777
- to stream Main, to step Curation: 005B38
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Experimental human metapneumovirus infection of cynomolgus macaques (Macaca fascicularis) results in virus replication in ciliated epithelial cells and pneumocytes with associated lesions throughout the respiratory tract</title><author><name sortKey="Kuiken, Thijs" sort="Kuiken, Thijs" uniqKey="Kuiken T" first="Thijs" last="Kuiken">Thijs Kuiken</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Virology, Erasmus Medical Center</s1><s2>Rotterdam</s2><s3>NLD</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Pays-Bas</country><placeName><settlement type="city">Rotterdam</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Van Den Hoogen, Bernadette G" sort="Van Den Hoogen, Bernadette G" uniqKey="Van Den Hoogen B" first="Bernadette G." last="Van Den Hoogen">Bernadette G. Van Den Hoogen</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Virology, Erasmus Medical Center</s1><s2>Rotterdam</s2><s3>NLD</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Pays-Bas</country><placeName><settlement type="city">Rotterdam</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Van Riel, Debby A J" sort="Van Riel, Debby A J" uniqKey="Van Riel D" first="Debby A. J." last="Van Riel">Debby A. J. Van Riel</name><affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Department of Immunology, Erasmus Medical Center</s1><s2>Rotterdam</s2><s3>NLD</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Pays-Bas</country><placeName><settlement type="city">Rotterdam</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Laman, Jon D" sort="Laman, Jon D" uniqKey="Laman J" first="Jon D." last="Laman">Jon D. Laman</name><affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Department of Immunology, Erasmus Medical Center</s1><s2>Rotterdam</s2><s3>NLD</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Pays-Bas</country><placeName><settlement type="city">Rotterdam</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Van Amerongen, Geert" sort="Van Amerongen, Geert" uniqKey="Van Amerongen G" first="Geert" last="Van Amerongen">Geert Van Amerongen</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Virology, Erasmus Medical Center</s1><s2>Rotterdam</s2><s3>NLD</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Pays-Bas</country><placeName><settlement type="city">Rotterdam</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Sprong, Leo" sort="Sprong, Leo" uniqKey="Sprong L" first="Leo" last="Sprong">Leo Sprong</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Virology, Erasmus Medical Center</s1><s2>Rotterdam</s2><s3>NLD</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Pays-Bas</country><placeName><settlement type="city">Rotterdam</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Fouchier, Ron A M" sort="Fouchier, Ron A M" uniqKey="Fouchier R" first="Ron A. M." last="Fouchier">Ron A. M. Fouchier</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Virology, Erasmus Medical Center</s1><s2>Rotterdam</s2><s3>NLD</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Pays-Bas</country><placeName><settlement type="city">Rotterdam</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Osterhaus, Albert D M E" sort="Osterhaus, Albert D M E" uniqKey="Osterhaus A" first="Albert D. M. E." last="Osterhaus">Albert D. M. E. Osterhaus</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Virology, Erasmus Medical Center</s1><s2>Rotterdam</s2><s3>NLD</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Pays-Bas</country><placeName><settlement type="city">Rotterdam</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">04-0317025</idno><date when="2004">2004</date><idno type="stanalyst">PASCAL 04-0317025 INIST</idno><idno type="RBID">Pascal:04-0317025</idno><idno type="wicri:Area/PascalFrancis/Corpus">000875</idno><idno type="wicri:Area/PascalFrancis/Curation">000115</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000849</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000849</idno><idno type="wicri:doubleKey">0002-9440:2004:Kuiken T:experimental:human:metapneumovirus</idno><idno type="wicri:Area/Main/Merge">006000</idno><idno type="wicri:source">PMC</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1615765</idno><idno type="RBID">PMC:1615765</idno><idno type="wicri:Area/Pmc/Corpus">000822</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000822</idno><idno type="wicri:Area/Pmc/Curation">000822</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000822</idno><idno type="wicri:Area/Pmc/Checkpoint">001666</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001666</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:15161626</idno><idno type="wicri:Area/PubMed/Corpus">002D81</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002D81</idno><idno type="wicri:Area/PubMed/Curation">002D81</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002D81</idno><idno type="wicri:Area/PubMed/Checkpoint">002D09</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002D09</idno><idno type="wicri:Area/Ncbi/Merge">000829</idno><idno type="wicri:Area/Ncbi/Curation">000829</idno><idno type="wicri:Area/Ncbi/Checkpoint">000829</idno><idno type="wicri:doubleKey">0002-9440:2004:Kuiken T:experimental:human:metapneumovirus</idno><idno type="wicri:Area/Main/Merge">005777</idno><idno type="wicri:Area/Main/Curation">005B38</idno><idno type="wicri:Area/Main/Exploration">005B38</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Experimental human metapneumovirus infection of cynomolgus macaques (Macaca fascicularis) results in virus replication in ciliated epithelial cells and pneumocytes with associated lesions throughout the respiratory tract</title><author><name sortKey="Kuiken, Thijs" sort="Kuiken, Thijs" uniqKey="Kuiken T" first="Thijs" last="Kuiken">Thijs Kuiken</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Virology, Erasmus Medical Center</s1><s2>Rotterdam</s2><s3>NLD</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Pays-Bas</country><placeName><settlement type="city">Rotterdam</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Van Den Hoogen, Bernadette G" sort="Van Den Hoogen, Bernadette G" uniqKey="Van Den Hoogen B" first="Bernadette G." last="Van Den Hoogen">Bernadette G. Van Den Hoogen</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Virology, Erasmus Medical Center</s1><s2>Rotterdam</s2><s3>NLD</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Pays-Bas</country><placeName><settlement type="city">Rotterdam</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Van Riel, Debby A J" sort="Van Riel, Debby A J" uniqKey="Van Riel D" first="Debby A. J." last="Van Riel">Debby A. J. Van Riel</name><affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Department of Immunology, Erasmus Medical Center</s1><s2>Rotterdam</s2><s3>NLD</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Pays-Bas</country><placeName><settlement type="city">Rotterdam</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Laman, Jon D" sort="Laman, Jon D" uniqKey="Laman J" first="Jon D." last="Laman">Jon D. Laman</name><affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Department of Immunology, Erasmus Medical Center</s1><s2>Rotterdam</s2><s3>NLD</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Pays-Bas</country><placeName><settlement type="city">Rotterdam</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Van Amerongen, Geert" sort="Van Amerongen, Geert" uniqKey="Van Amerongen G" first="Geert" last="Van Amerongen">Geert Van Amerongen</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Virology, Erasmus Medical Center</s1><s2>Rotterdam</s2><s3>NLD</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Pays-Bas</country><placeName><settlement type="city">Rotterdam</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Sprong, Leo" sort="Sprong, Leo" uniqKey="Sprong L" first="Leo" last="Sprong">Leo Sprong</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Virology, Erasmus Medical Center</s1><s2>Rotterdam</s2><s3>NLD</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Pays-Bas</country><placeName><settlement type="city">Rotterdam</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Fouchier, Ron A M" sort="Fouchier, Ron A M" uniqKey="Fouchier R" first="Ron A. M." last="Fouchier">Ron A. M. Fouchier</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Virology, Erasmus Medical Center</s1><s2>Rotterdam</s2><s3>NLD</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Pays-Bas</country><placeName><settlement type="city">Rotterdam</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Osterhaus, Albert D M E" sort="Osterhaus, Albert D M E" uniqKey="Osterhaus A" first="Albert D. M. E." last="Osterhaus">Albert D. M. E. Osterhaus</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Virology, Erasmus Medical Center</s1><s2>Rotterdam</s2><s3>NLD</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Pays-Bas</country><placeName><settlement type="city">Rotterdam</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author></analytic><series><title level="j" type="main">The American journal of pathology</title><title level="j" type="abbreviated">Am. j. pathol.</title><idno type="ISSN">0002-9440</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">The American journal of pathology</title><title level="j" type="abbreviated">Am. j. pathol.</title><idno type="ISSN">0002-9440</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Association</term><term>Bronchi (pathology)</term><term>Bronchi (virology)</term><term>Cilia (virology)</term><term>Ciliated cell</term><term>Disease Models, Animal</term><term>Epithelial Cells (ultrastructure)</term><term>Epithelial Cells (virology)</term><term>Epithelial cell</term><term>Experimental study</term><term>Human</term><term>Humans</term><term>Infection</term><term>Lesion</term><term>Lung (pathology)</term><term>Lung (virology)</term><term>Macaca fascicularis</term><term>Medicine</term><term>Metapneumovirus (isolation & purification)</term><term>Metapneumovirus (physiology)</term><term>Monkey</term><term>Paramyxoviridae Infections (pathology)</term><term>Paramyxoviridae Infections (physiopathology)</term><term>Paramyxoviridae Infections (virology)</term><term>Pneumocyte</term><term>Replication</term><term>Respiratory Tract Infections (pathology)</term><term>Respiratory Tract Infections (physiopathology)</term><term>Respiratory Tract Infections (virology)</term><term>Respiratory tract</term><term>Trachea (pathology)</term><term>Trachea (virology)</term><term>Virus</term><term>Virus Replication (physiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Bronches (anatomopathologie)</term><term>Bronches (virologie)</term><term>Cellules épithéliales (ultrastructure)</term><term>Cellules épithéliales (virologie)</term><term>Cils vibratiles (virologie)</term><term>Humains</term><term>Infections de l'appareil respiratoire (anatomopathologie)</term><term>Infections de l'appareil respiratoire (physiopathologie)</term><term>Infections de l'appareil respiratoire (virologie)</term><term>Infections à Paramyxoviridae (anatomopathologie)</term><term>Infections à Paramyxoviridae (physiopathologie)</term><term>Infections à Paramyxoviridae (virologie)</term><term>Macaca fascicularis</term><term>Metapneumovirus (isolement et purification)</term><term>Metapneumovirus (physiologie)</term><term>Modèles animaux de maladie humaine</term><term>Poumon (anatomopathologie)</term><term>Poumon (virologie)</term><term>Réplication virale (physiologie)</term><term>Trachée (anatomopathologie)</term><term>Trachée (virologie)</term></keywords><keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Bronches</term><term>Infections de l'appareil respiratoire</term><term>Infections à Paramyxoviridae</term><term>Poumon</term><term>Trachée</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Metapneumovirus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Metapneumovirus</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Bronchi</term><term>Lung</term><term>Paramyxoviridae Infections</term><term>Respiratory Tract Infections</term><term>Trachea</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Metapneumovirus</term><term>Réplication virale</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Metapneumovirus</term><term>Virus Replication</term></keywords><keywords scheme="MESH" qualifier="physiopathologie" xml:lang="fr"><term>Infections de l'appareil respiratoire</term><term>Infections à Paramyxoviridae</term></keywords><keywords scheme="MESH" qualifier="physiopathology" xml:lang="en"><term>Paramyxoviridae Infections</term><term>Respiratory Tract Infections</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Epithelial Cells</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Animaux</term><term>Bronches</term><term>Cellules épithéliales</term><term>Cils vibratiles</term><term>Humains</term><term>Infections de l'appareil respiratoire</term><term>Infections à Paramyxoviridae</term><term>Macaca fascicularis</term><term>Modèles animaux de maladie humaine</term><term>Poumon</term><term>Trachée</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Bronchi</term><term>Cilia</term><term>Epithelial Cells</term><term>Lung</term><term>Paramyxoviridae Infections</term><term>Respiratory Tract Infections</term><term>Trachea</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Disease Models, Animal</term><term>Humans</term><term>Macaca fascicularis</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Infection</term><term>Etude expérimentale</term><term>Homme</term><term>Singe</term><term>Macaca fascicularis</term><term>Virus</term><term>Réplication</term><term>Cellule ciliée</term><term>Cellule épithéliale</term><term>Pneumocyte</term><term>Association</term><term>Lésion</term><term>Voie respiratoire</term><term>Médecine</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Homme</term><term>Singe</term><term>Association</term><term>Médecine</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A substantial proportion of hitherto unexplained respiratory tract illnesses is associated with human metapneumovirus (hMPV) infection. This virus also was found in patients with severe acute respiratory syndrome (SARS). To determine the dynamics and associated lesions of hMPV infection, six cynomolgus macaques (Macaca fascicularis) were inoculated with hMPV and examined by pathological and virological assays. They were euthanized at 5 (n = 2) or 9 (n = 2) days post-infection (dpi), or monitored until 14 dpi (n = 2). Viral excretion peaked at 4 dpi and decreased to zero by 10 dpi. Viral replication was restricted to the respiratory tract and associated with minimal to mild, multi-focal erosive and inflammatory changes in conducting airways, and increased numbers of macrophages in alveoli. Viral expression was seen mainly at the apical surface of ciliated epithelial cells throughout the respiratory tract, and less frequently in type 1 pneumocytes and alveolar macrophages. Both cell tropism and respiratory lesions were distinct from those of SARS-associated coronavirus infection, excluding hMPV as the primary cause of SARS. This study demonstrates that hMPV is a respiratory pathogen and indicates that viral replication is short-lived, polarized to the apical surface, and occurs primarily in ciliated respiratory epithelial cells.</div></front></TEI><affiliations><list><country><li>Pays-Bas</li></country><region><li>Hollande-Méridionale</li></region><settlement><li>Rotterdam</li></settlement></list><tree><country name="Pays-Bas"><region name="Hollande-Méridionale"><name sortKey="Kuiken, Thijs" sort="Kuiken, Thijs" uniqKey="Kuiken T" first="Thijs" last="Kuiken">Thijs Kuiken</name></region><name sortKey="Fouchier, Ron A M" sort="Fouchier, Ron A M" uniqKey="Fouchier R" first="Ron A. M." last="Fouchier">Ron A. M. Fouchier</name><name sortKey="Laman, Jon D" sort="Laman, Jon D" uniqKey="Laman J" first="Jon D." last="Laman">Jon D. Laman</name><name sortKey="Osterhaus, Albert D M E" sort="Osterhaus, Albert D M E" uniqKey="Osterhaus A" first="Albert D. M. E." last="Osterhaus">Albert D. M. E. Osterhaus</name><name sortKey="Sprong, Leo" sort="Sprong, Leo" uniqKey="Sprong L" first="Leo" last="Sprong">Leo Sprong</name><name sortKey="Van Amerongen, Geert" sort="Van Amerongen, Geert" uniqKey="Van Amerongen G" first="Geert" last="Van Amerongen">Geert Van Amerongen</name><name sortKey="Van Den Hoogen, Bernadette G" sort="Van Den Hoogen, Bernadette G" uniqKey="Van Den Hoogen B" first="Bernadette G." last="Van Den Hoogen">Bernadette G. Van Den Hoogen</name><name sortKey="Van Riel, Debby A J" sort="Van Riel, Debby A J" uniqKey="Van Riel D" first="Debby A. J." last="Van Riel">Debby A. J. Van Riel</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 005B38 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 005B38 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= Pascal:04-0317025
|texte= Experimental human metapneumovirus infection of cynomolgus macaques (Macaca fascicularis) results in virus replication in ciliated epithelial cells and pneumocytes with associated lesions throughout the respiratory tract
}}
| This area was generated with Dilib version V0.6.33. |  |